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RT-LAMP could be used for SARS-CoV-2 testing and overcomes the challenges of adapting an assay to a point-of-care instrument. Further, the reduced instrumentation cost and complexity, along with the simple workflow, highlight the potential for implementation of RT-LAMP for SARS-CoV-2 testing in resource-limited settings.
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Jung-Chung Lin b Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei,. In particular, using real-time RT-PCR with the lab-developed PCR assay and the BD Max system, we detected 55 samples as positive and 150 samples as negative for SARS-CoV-2..
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The developed protocol was applied to one-step dPCR, which allowed the detection of 1,130.2 RNA copies/µL using 10 −2 μg/nL of viral particles with a 30 min thermolysis-RT reaction at 70°C ( Table 5 ). The protocol developed by optimizing RT-qPCR analysis was verified and applied to dPCR analysis.
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Analysis of the results, in comparison to RT-qPCR, found that the ZIKV RT-LAMP assay provided sensitivity of 100%, specificity of 93.75%, and an overall accuracy of 95.00%.
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Schematics of RT-LAMP amplification, exemplified for SARS-CoV-2detection.. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a one step nucleic acid amplification method to multiply specific sequences of RNA.It is used to diagnose infectious disease caused by RNA viruses.. It combines LAMP DNA-detection with reverse transcription, making cDNA from RNA before running the.
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The global COVID-19 pandemic has highlighted the need for rapid, accurate and accessible nucleic acid tests to enable timely identification of infected individuals. We optimized a sample-to-answer nucleic acid test for SARS-CoV-2 that provides results in <1 hour using inexpensive and readily available reagents. The test workflow includes a simple lysis and viral inactivation protocol followed.
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In 2019 a newly identified coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has spread rapidly from the epicenter in Wuhan (China) to more than 150 countries around the world, causing the Coronavirus disease 2019 (COVID-19) pandemic. In this study, we describe an extraction-less method based on reverse transcriptase loop-mediated isothermal amplification.
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In this field trial, RT-LAMP appears to be a reliable assay, comparable to RT-PCR, particularly with medium-high viral loads (Ct < 35). Hence, RT-LAMP can be deployed in RLS for timely management and prevention of COVID-19, without compromising the quality of output.
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The RNAzol RT reagent was used to provide pure RNA from human cells. We develop a protocol using RNAzol RT reagent to extract pure RNA from plants tissues and demonstrate that this RNA extraction method works not only at room temperature but also at elevated temperatures and provides the simplest and most effective single-step method to extract pure and undegraded RNA directly from tropical.
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Plant materials. To develop single and multiplex RT-LAMP assays, we collected plant material from various Musa genotypes growing at the Waimanalo Research Station, College of Tropical Agriculture and Human Resources (CTAHR), University of Hawaii at Manoa, located in Honolulu County, HI, USA. The material included twelve samples from plants with typical symptoms of banana bunchy top disease.
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Background Children are the most vulnerable group affected by malaria and other tropical, vector-borne diseases in low-resource countries. Infants presenting with acute onset fever represent a major sector of outpatient care in the Lake Victoria region. Misclassification and overuse of antibiotics and anti-malarial medications are consistent problems. Identifying the prevalent mosquito-borne.